TASP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TASP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16739-1-AP

Synonyms

Threonine aspartase subunit alpha, Threonine aspartase 1, Taspase-1, Taspase 1, EC:3.4.25.-

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Validation Data Gallery

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  WB analysis of HEK-293 using 16739-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16739-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16739-1-AP

  Immunofluorescent analysis of HeLa cells using 16739-1-AP (TASP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human ovary tissue, human kidney tissue, human liver tissue, human lung tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16739-1-AP targets TASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TASP1 fusion protein Ag9918
• Full Name: taspase, threonine aspartase, 1
• Calculated Molecular Weight: 420 aa, 44 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC025266
• Gene Symbol: TASP1
• Gene ID (NCBI): 55617
• RRID: AB_2201218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Suleiman-El-Hattab syndrome: a histone modification disorder caused by TASP1 deficiency.; Authors - Korbinian M Riedhammer ; KD Validated
human	WB,IHC	Cancer Manag Res; Overexpression of Taspase 1 Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma.; Authors - Jie Jiang