Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human ovary tissue, human kidney tissue, human liver tissue, human lung tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
16739-1-AP targets TASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9918 Product name: Recombinant human TASP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-420 aa of BC025266 Sequence: MTMEKGMSSGEGLPSRSSQVSAGKITAKELETKQSYKEKRGGFVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAGMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPPCFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQSSEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHNPYSTAVSTSGCGEHLVRTILARECSHALQAEDAHQALLETMQNKFISSPFLASEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEFLWSHTTESMCVGYMSAQDGKAKTHISRLPPGAVAGQSVAIEGGVCRLESPVN Predict reactive species |
| Full Name | taspase, threonine aspartase, 1 |
| Calculated Molecular Weight | 420 aa, 44 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC025266 |
| Gene Symbol | TASP1 |
| Gene ID (NCBI) | 55617 |
| RRID | AB_2201218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Threonine aspartase 1 (TASP1), a particularly conserved protease that cleaves mixed lineage leukemia 1 (MLL), regulates the expression of homeotic genes and is involved in the occurrence of cancer. TASP1 drives cell cycle progression and coordinates cell proliferation and apoptosis (PMID: 33767369). Aberrant expression of TASP1 has been found in numerous liquid and solid malignancies, including leukaemia, breast cancer, lung cancer, head and neck cancer, brain cancer, colon cancer, glioblastoma and melanoma. TASP1 plays a key role in cancer cell proliferation and apoptosis and can inhibit tumor growth after knockout (PMID: 32071545, 34012990).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TASP1 antibody 16739-1-AP | Download protocol |
| IHC protocol for TASP1 antibody 16739-1-AP | Download protocol |
| WB protocol for TASP1 antibody 16739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Suleiman-El-Hattab syndrome: a histone modification disorder caused by TASP1 deficiency.
| ||
Cancer Manag Res Overexpression of Taspase 1 Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma. |
