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TAU Recombinant monoclonal antibody, PBS Only (82846-5-PBS)

TAU Recombinant monoclonal antibody, PBS Only

Name: TAU Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 82846-5-PBS-100UG

TAU Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 82846-5-PBS

Clone No.252452C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBIHCIF-PIF-FroAffinity

WB analysis using 82846-5-RR (same clone as 82846-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82846-5-RR (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 82846-5-RR
Biolayer interferometry (BLI) kinetic assays of 82846-5-RR against Human TAU were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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Product Information

82846-5-PBS targets TAU in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6232 Product name: Recombinant human TAU protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1-758 aa of NM_016835.4 Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL Predict reactive species
Full Name	microtubule-associated protein tau
Calculated Molecular Weight	79 kDa
Observed Molecular Weight	45-70 kDa
GenBank Accession Number	NM_016835.4
Gene Symbol	TAU
Gene ID (NCBI)	4137
RRID	AB_3743441
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10636
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697).