Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
11692-1-AP targets TAX1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2306 Product name: Recombinant human TAX1BP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-124 aa of BC023980 Sequence: MSYIPGQPVTAVVQRVEIHKLRQGENLILGFSIGGGIDQDPSQNPFSEDKTDKGIYVTRVSEGGPAEIAGLQIGDKIMQVNGWDMTMVTHDQARKRLTKRSEEVVRLLVTRQSLQKAVQQSMLS Predict reactive species |
| Full Name | Tax1 (human T-cell leukemia virus type I) binding protein 3 |
| Calculated Molecular Weight | 124 aa, 14 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC023980 |
| Gene Symbol | TAX1BP3 |
| Gene ID (NCBI) | 30851 |
| RRID | AB_11182823 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tax-interacting protein 1 (TIP-1, also known as Tax1bp3 or glutaminase-interacting protein, GIP) is unique in its simple structure, a single PDZ domain is the only functional and structural unit identified so far in this small PDZ protein (124 amino acids in human and mouse). High level of TIP-1 expression was detected in human invasive breast cancer cells and that is important for tumor cell adhesion, migration and pulmonary metastasis. However, the precise biological effects of TIP-1 and its functional mechanisms have largely remained elusive. Mutation of TAX1BP3 can cause Dilated Cardiomyopathy with Septo-Optic Dysplasia (PMID: 25645515).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TAX1BP3 antibody 11692-1-AP | Download protocol |
| WB protocol for TAX1BP3 antibody 11692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CHAO (Verified Customer) (03-31-2022) | Good clear bands.
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FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
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FH Jie (Verified Customer) (01-23-2020) | works well with a specific band that matches the product size
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FH Canzhao (Verified Customer) (01-17-2020) | It is one of the best antibody I used. It is very specific. I would like to give it 6 stars if it is possible.
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