TAX1BP3 Polyclonal antibody

TAX1BP3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (4)

Cat no : 11692-1-AP

Synonyms

Tax interaction protein 1, Tax1 binding protein 3, TAX1BP3, TIP 1, TIP1

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Validation Data Gallery

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  WB analysis using 11692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11692-1-AP (TAX1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11692-1-AP targets TAX1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAX1BP3 fusion protein Ag2306
• Full Name: Tax1 (human T-cell leukemia virus type I) binding protein 3
• Calculated Molecular Weight: 124 aa, 14 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC023980
• Gene Symbol: TAX1BP3
• Gene ID (NCBI): 30851
• RRID: AB_11182823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tax-interacting protein 1 (TIP-1, also known as Tax1bp3 or glutaminase-interacting protein, GIP) is unique in its simple structure, a single PDZ domain is the only functional and structural unit identified so far in this small PDZ protein (124 amino acids in human and mouse). High level of TIP-1 expression was detected in human invasive breast cancer cells and that is important for tumor cell adhesion, migration and pulmonary metastasis. However, the precise biological effects of TIP-1 and its functional mechanisms have largely remained elusive. Mutation of TAX1BP3 can cause Dilated Cardiomyopathy with Septo-Optic Dysplasia (PMID: 25645515).

Publications

Species	Application	Title
human,mouse	WB,IHC	Gene; Birc3 and Tip1 are upregulated in renal ischemia reperfusion injury; Authors - Sixu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Good clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-23-2020)

works well with a specific band that matches the product size

• Applications: Western Blot,
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Human kidney biopsy

FH

Canzhao (Verified Customer) (01-17-2020)

It is one of the best antibody I used. It is very specific. I would like to give it 6 stars if it is possible.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.