TBC1D17 Polyclonal antibody

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• TBC1D17 Polyclonal antibody (20482-1-AP)

TBC1D17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20482-1-AP Copied!

Publications (3)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TBC1 domain family member 17, TBC1 domain family, member 17

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  WB analysis using 20482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 20482-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 20482-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 20482-1-AP

  IP result of anti-TBC1D17 (IP:20482-1-AP, 4ug; Detection:20482-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 20482-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20482-1-AP (TBC1D17 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse thymus tissue, Jurkat cells, rat brain tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human heart tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20482-1-AP targets TBC1D17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14358

  Product name: Recombinant human TBC1D17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC003516

  Sequence: MEGAGYRVVFEKGGVYLHTSAKKYQDRDSLIAGVIRVVEKDNDVLLHWAPVEEAGDSTQILFSKKDSSGGDSCASEEEPTFDPGYEPDWAVISTVRPQPCHSEPTRGAEPSCPQGSWAFSVSLGELKSIRRSKPGLSWAYLVLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPHDSSALSNSFHHLQLFDQDSSNVVSRFLQDPYSTTFSSFSRVTNFFRGALQPQPEGAASDLPPPPDDEPEPGFEVISCVELGPRPTVERGPPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAEEHKAHIRKKTDEYFRM

• Full Name: TBC1 domain family, member 17
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC003516
• Gene Symbol: TBC1D17
• Gene ID (NCBI): 79735
• RRID: AB_11232420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HA65
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1 domain family member 17 (TBC1D17), a Rab GTPase-activating protein, through its catalytic activity, regulates Rab8-mediated endocytic trafficking of transferrin receptor (PMID: 22854040). TBC1D17 also inhibits the trafficking of Shiga toxin from plasma membrane to the Golgi complex through its catalytic activity (PMID: 1756278).

Publications

What published studies show

The TBC1D17 Polyclonal antibody (Cat# 20482-1-AP) has 3 citations. They appear in Science Bulletin, Cell Death & Disease, and Cell Reports. The research spans hepatitis B virus–driven liver disease, hypoxia-induced mitophagy and cell survival (SENP3-FIS1 axis), and mitochondrial regulation of STING degradation via Rab7A. Cited applications include WB and IF in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Sci Bull (Beijing); Hepatitis B virus core protein as a Rab-GAP suppressor driving liver disease progression; Authors - Yu Su
human	WB,IF	Cell Death Dis; SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia; Authors - Alice Zhao
		Cell Rep; Mitochondrial Fis1 tunes Rab7A activity via TBC1D15 modulating STING degradation.; Authors - Eunice Dominguez-Martin

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single review for this product reports a 4-star rating from a Western Blot experiment performed on HeLa cells at a 1:1,000 dilution. The reviewer simply stated that the antibody worked well, indicating satisfactory performance for their application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells