TBC1D17 Monoclonal antibody

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  WB analysis using 60975-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60975-3-Ig (TBC1D17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60975-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60975-3-Ig (TBC1D17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TBC1D17 Monoclonal Antibody for WB, ELISA

Cat No. 60975-3-Ig

Clone No.1H3C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rabbit

Applications

WB, ELISA

Synonyms

TBC1 domain family member 17, TBC1 domain family, member 17

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Tested Applications

• Positive WB detected in: A549 cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, A172 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60975-3-Ig targets TBC1D17 in WB, ELISA applications and shows reactivity with human, pig, rabbit samples.

• Tested Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14358

  Product name: Recombinant human TBC1D17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC003516

  Sequence: MEGAGYRVVFEKGGVYLHTSAKKYQDRDSLIAGVIRVVEKDNDVLLHWAPVEEAGDSTQILFSKKDSSGGDSCASEEEPTFDPGYEPDWAVISTVRPQPCHSEPTRGAEPSCPQGSWAFSVSLGELKSIRRSKPGLSWAYLVLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPHDSSALSNSFHHLQLFDQDSSNVVSRFLQDPYSTTFSSFSRVTNFFRGALQPQPEGAASDLPPPPDDEPEPGFEVISCVELGPRPTVERGPPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAEEHKAHIRKKTDEYFRM

• Full Name: TBC1 domain family, member 17
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC003516
• Gene Symbol: TBC1D17
• Gene ID (NCBI): 79735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HA65
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1 domain family member 17 (TBC1D17), a Rab GTPase-activating protein, through its catalytic activity, regulates Rab8-mediated endocytic trafficking of transferrin receptor (PMID: 22854040). TBC1D17 also inhibits the trafficking of Shiga toxin from plasma membrane to the Golgi complex through its catalytic activity (PMID: 1756278).