TBC1D19 Polyclonal antibody

TBC1D19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21085-1-AP

Synonyms

TBC1 domain family member 19, TBC1 domain family, member 19, TBC1D19

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Validation Data Gallery

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  WB analysis of mouse brain using 21085-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21085-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21085-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21085-1-AP (TBC1D19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 21085-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21085-1-AP (TBC1D19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 21085-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21085-1-AP (TBC1D19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21085-1-AP

  Immunofluorescent analysis of HepG2 cells using 21085-1-AP (TBC1D19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21085-1-AP is TBC1D19 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D19 fusion protein Ag15323
• Full Name: TBC1 domain family, member 19
• Calculated Molecular Weight: 526 aa, 60 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC031642
• Gene Symbol: TBC1D19
• Gene ID (NCBI): 55296
• RRID: AB_2878809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TBC1D19 remains largely unknown. TBC1D19 may act as a GTPase-activating protein for Rab family protein(s).