TBC1D20 Polyclonal antibody

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  WB analysis using 16464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16464-1-AP

  WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

• 

  WB analysis of A549 using 16464-1-AP

  WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

TBC1D20 Polyclonal Antibody for WB, ELISA

Cat No. 16464-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C20orf140, TBC1 domain family member 20, TBC1 domain family, member 20, TBC1D20

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16464-1-AP targets TBC1D20 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9539

  Product name: Recombinant human TBC1D20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC014983

  Sequence: MALRSAQGDGPTSGHWDGGAEKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRRKVWPKLLNVNANDPPPISGKNLRQMSKDYQQVLLDVRRSLRRFPPGMPEEQREGLQEELIDIILLILERNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLSDFRHVVRLYDFFLACHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQQAERTAASTFKDFELASAQQRPDMVLRQRFRGLLRPEDRTKDVLTKPRTNR

• Full Name: TBC1 domain family, member 20
• Calculated Molecular Weight: 403 aa, 46 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC014983
• Gene Symbol: TBC1D20
• Gene ID (NCBI): 128637
• RRID: AB_3669245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96BZ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D20 belongs to a family of GTPase activator proteins of Rab-like small GTPases.TBC1D20 is an endoplasmic reticulum (ER) type II membrane protein with the TBC domain positioned in the cytosol. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4.