TBC1D23 Polyclonal antibody

• Featured Product
• KD/KO Validated

TBC1D23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 17002-1-AP

Synonyms

TBC1 domain family, member 23, TBC1 domain family member 23, NS4ATP1, HCV non-structural protein 4A-transactivated protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 17002-1-AP

  WB result of TBC1D23 antibody (17002-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D23 transfected HEK-293 cells.

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  WB analysis of HepG2 using 17002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17002-1-AP

  IP result of anti-TBC1D23 (IP:17002-1-AP, 4ug; Detection:17002-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17002-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 17002-1-AP (TBC1D23 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

17002-1-AP targets TBC1D23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D23 fusion protein Ag10697
• Full Name: TBC1 domain family, member 23
• Calculated Molecular Weight: 699 aa, 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC010221
• Gene Symbol: TBC1D23
• Gene ID (NCBI): 55773
• RRID: AB_2240206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.

Publications

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
	WB	Nat Commun; TBC1D23 mediates Golgi-specific LKB1 signaling; Authors - Yingfeng Tu
human	WB,IF	Am J Hum Genet; Homozygous Mutations in TBC1D23 Lead to a Non-degenerative Form of Pontocerebellar Hypoplasia.; Authors - Isaac Marin-Valencia
human	IF	Am J Hum Genet; Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development.; Authors - Ekaterina L Ivanova
human	WB,IHC	Int J Cancer; Identification of frequently mutated genes with relevance to nonsense mediated mRNA decay in the high microsatellite instability cancers.; Authors - Shin Nara N
human	IHC,WB,IF	J Cell Mol Med; TBC1 domain family member 23 interacts with Ras-related protein Rab-11A to promote poor prognosis of non-small-cell lung cancer via β1-integrin.; Authors - Yao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Main band detected below the 85 kDa marker (expected size of TBC1D23 is 78, observed is 70-78).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T

FH

Jerome (Verified Customer) (05-22-2019)

Most cells show the expected Golgi localisation; but not all. High background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

TBC1D23 Antibody Immunofluorescence, validation (1:100 dilution) in HeLa cells (Cat no:17002-1-AP)