Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human stomach tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25254-1-AP targets TBC1D24 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19380 Product name: Recombinant human TBC1D24 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-321 aa of BC127015 Sequence: MDSPGYNCFVDKDKMDAAIQDLGPKELSCTELQELKQLARQGYWAQSHALRGKVYQRLIRDIPCRTVTPDASVYSDIVGKIVGKHSSSCLPLPEFVDNTQVPSYCLNARGEGAVRKILLCLANQFPDISFCPALPAVVALLLHYSIDEAECFEKACRILACNDPGRRLIDQSFLAFESSCMTFGDLVNKYCQAAHKLMVAVSEDVLQVYADWQRWLFGELPLCYFARVFDVFLVEGYKVLYRVALAILKFFHKVRAGQPLESDSVKQDIRTFVRDIAKTVSPEKLLEKAFAIRLFSRKEIQLLQMANEKALKQKGITVKQK Predict reactive species |
| Full Name | TBC1 domain family, member 24 |
| Calculated Molecular Weight | 559 aa, 63 kDa |
| Observed Molecular Weight | 60-63 kDa |
| GenBank Accession Number | BC127015 |
| Gene Symbol | TBC1D24 |
| Gene ID (NCBI) | 57465 |
| RRID | AB_2879990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULP9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBC1D24 antibody 25254-1-AP | Download protocol |
| WB protocol for TBC1D24 antibody 25254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBC1D24 Polyclonal antibody (catalog number 25254-1-AP) has a total of 2 citations. These appear in Cell Mol Life Sci and PLoS One. The associated research fields involve neurodevelopment and behavior, V-ATPase formation and function, and neuronal cellular juvenescence (cytophidia). Applications cited include Western blot (WB) and immunohistochemistry (IHC), with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
Reviews
What customers say
The single review for this antibody is highly positive, with a 5-star rating. The reviewer used it for Western Blot at a 1:2000 dilution on human postmortem brain lysate and described it as a good antibody for detecting TBC1D24. Overall, the feedback indicates reliable performance for its intended target in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xin (Verified Customer) (03-15-2022) | A good antibody to detect TBC1D24
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