TBC1D2B Polyclonal antibody

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• TBC1D2B Polyclonal antibody (24462-1-AP)

• 

  WB analysis using 24462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24462-1-AP (TBC1D2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 24462-1-AP

  WB result of TBC1D2B antibody (24462-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D2B transfected U-251 cells.

• 

  IP experiment of BxPC-3 using 24462-1-AP

  IP result of anti-TBC1D2B (IP:24462-1-AP, 4ug; Detection:24462-1-AP 1:600) with BxPC-3 cells lysate 1200 ug.

• KD/KO Validated

TBC1D2B Polyclonal Antibody for WB, IP, ELISA

Cat No. 24462-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

TBC1 domain family member 2B, TBC1 domain family, member 2B

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Tested Applications

• Positive WB detected in: BxPC-3 cells, U-251 cells, HL-60 cells, mouse spleen tissue
• Positive IP detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24462-1-AP targets TBC1D2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19632

  Product name: Recombinant human TBC1D2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 324-655 aa of BC152465

  Sequence: KDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEV

• Full Name: TBC1 domain family, member 2B
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC152465
• Gene Symbol: TBC1D2B
• Gene ID (NCBI): 23102
• RRID: AB_3742115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPU7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1 domain family member 2B (TBC1D2B) contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP and ATG12 conjugation complexes (PMID: 38226533). When there is a high protein level of RAB22A on early endosome, TBC1D2B is recruited and acts as a brake to prevent early endosomes from maturing into late endosomes (PMID: 39051763).