TBC1D5 Recombinant antibody

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  WB analysis using 84990-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84990-4-RR (TBC1D5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84990-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBC1D5 antibody (84990-4-RR, Clone: 242534B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84990-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84990-4-RR against Human TBC1D5 were performed. The affinity constant is 3.28 nM.

TBC1D5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84990-4-RR

Clone No.242534B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242534B8, KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-3 cells, A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84990-4-RR targets TBC1D5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBC1D5 fusion protein Ag10991
• Full Name: TBC1 domain family, member 5
• Calculated Molecular Weight: 795 aa, 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC013145
• Gene Symbol: TBC1D5
• Gene ID (NCBI): 9779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.