TBCC Polyclonal antibody

TBCC Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15917-1-AP

Synonyms

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC

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Validation Data Gallery

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  WB analysis of HepG2 using 15917-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15917-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15917-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15917-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15917-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15917-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15917-1-AP (TBCC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15917-1-AP

  Immunofluorescent analysis of HepG2 cells, using TBCC antibody 15917-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, HeLa cells, K-562 cells, MCF-7 cells, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15917-1-AP targets TBCC in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBCC fusion protein Ag8719
• Full Name: tubulin folding cofactor C
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC020170
• Gene Symbol: TBCC
• Gene ID (NCBI): 6903
• RRID: AB_2199538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).

Publications

Species	Application	Title
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens