TBK1 Polyclonal antibody

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• TBK1 Polyclonal antibody (28397-1-AP)

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• KD/KO Validated

TBK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 28397-1-AP Copied!

Publications (154)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1, Serine/threonine-protein kinase TBK1

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  WB analysis using 28397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis using 28397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28397-1-AP (TBK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 28397-1-AP

  WB result of TBK1 antibody (28397-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and TBK1 knockout HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HepG2 using 28397-1-AP

  IP result of anti-TBK1 (IP:28397-1-AP, 4ug; Detection:28397-1-AP 1:1000) with HepG2 cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of U2OS using 28397-1-AP

  U2OS lysates prepared and IP of TBK1 performed using 1.0 μg of 28397-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human liver cancer using 28397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human stomach cancer using 28397-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 28397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 28397-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 28397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28397-1-AP (TBK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 28397-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (28397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 28397-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBK1 antibody (28397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-1080 cells, HCT 116 cells, HeLa cells, HepG2 cells, U2OS cells
• Positive IP detected in: HepG2 cells, U2OS cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28397-1-AP targets TBK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28894

  Product name: Recombinant human TBK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 316-576 aa of BC034950

  Sequence: LQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA

• Full Name: TANK-binding kinase 1
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC034950
• Gene Symbol: TBK1
• Gene ID (NCBI): 29110
• RRID: AB_2881132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

Publications

What published studies show

The TBK1 Polyclonal antibody (catalog number 28397-1-AP) has 154 citations. These appear in journals including Signal Transduction and Targeted Therapy, Nature Communications, Cell Reports, Science Advances, Cancer Research, Molecular Cell, PLoS Pathogens, Journal of Virology, Frontiers in Immunology, and International Journal of Biological Sciences. Research fields span the cGAS-STING innate immune pathway, antiviral responses, cancer immunotherapy, neuroinflammation, ferroptosis, and inflammatory diseases, with applications mainly in Western blot, immunoprecipitation, and immunohistochemistry.

Automatically generated from the 154 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response.; Authors - Yinghua Zhao
mouse,human	WB	Signal Transduct Target Ther; The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation; Authors - Weijie Ouyang
		Cell Mol Immunol; Dynamic regulation of TBK1 lactylation shapes antiviral immune responses.; Authors - Yingchao Xie
mouse	WB	Bioact Mater; Targeting and reprogramming microglial phagocytosis of neutrophils by ginsenoside Rg1 nanovesicles promotes stroke recovery; Authors - Kaichao Hu
human	WB	Cancer Res; CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer; Authors - Wei Li
human	WB	Cancer Res; An Immune Subtype Classification System Enables the Development of Strategies to Predict and Enhance Immunotherapy Responses in Colorectal Cancer; Authors - Dingchang Li