Tested Applications
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13540-1-AP targets TBL1X in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4477 Product name: Recombinant human TBL1X protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC032708 Sequence: MSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPAALISILQKGLQYVEAEISINEDGTVFDGRPIESLSLIDAVMPDVVQTRQQAFREKLAQQQASAAAAAAAATAAATAATTTSAGVSHQNPSKNREATVNGEENRAHSVNNHAKPMEIDGEVEIPSSKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNTNGTLLATGSYDGFARIWTEDGNLASTLGQHKGPIFALKWNRKGNYILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGHTN Predict reactive species |
| Full Name | transducin (beta)-like 1X-linked |
| Calculated Molecular Weight | 526 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC032708 |
| Gene Symbol | TBL1X |
| Gene ID (NCBI) | 6907 |
| RRID | AB_2199783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TBL1X is an essential subunit of the nuclear receptor corepressor (NCoR)-silencing mediator for retinoid and thyroid hormone receptors (SMRT) complex, the major TH receptor (TR) corepressor (CoR) involved in T3-regulated gene expression. TBL1X defects were recently associated with central hypothyroidism and hearing loss. (PMID: 30591955, PMID: 27603907)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBL1X antibody 13540-1-AP | Download protocol |
| WB protocol for TBL1X antibody 13540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody targeting TBL1X/TBL1XR1 (Cat# 13540-1-AP) has 8 citations published in journals including Signal Transduction and Targeted Therapy, Nature Communications, International Journal of Biological Sciences, Cell Chemical Biology, Molecular Metabolism, FASEB Journal, and Development. Research fields span cancer biology (HDAC inhibitor efficacy, nasopharyngeal carcinoma metastasis), β-cell identity, trophoblast cell fate, atherosclerosis, brown adipose tissue thermogenesis, histone acetylation/gene regulation, and mammalian development.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Nat Commun TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network. | ||
Int J Biol Sci TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.
| ||
Cell Chem Biol HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation | ||
Mol Metab Nuclear receptor co-factor TBL1X/TBL1XR1 T cell activity protects against atherosclerosis.
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