TBL1X Polyclonal antibody

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  WB analysis of mouse pancreas using 13540-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13540-1-AP (TBL1X antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13540-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13540-1-AP (TBL1X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13540-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13540-1-AP (TBL1X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TBL1X Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13540-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Synonyms

EBI, F-box-like/WD repeat-containing protein TBL1X, SMAP55, TBL1, Transducin beta-like protein 1X

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

13540-1-AP targets TBL1X in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4477

  Product name: Recombinant human TBL1X protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-362 aa of BC032708

  Sequence: MSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPAALISILQKGLQYVEAEISINEDGTVFDGRPIESLSLIDAVMPDVVQTRQQAFREKLAQQQASAAAAAAAATAAATAATTTSAGVSHQNPSKNREATVNGEENRAHSVNNHAKPMEIDGEVEIPSSKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNTNGTLLATGSYDGFARIWTEDGNLASTLGQHKGPIFALKWNRKGNYILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGHTN

• Full Name: transducin (beta)-like 1X-linked
• Calculated Molecular Weight: 526 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC032708
• Gene Symbol: TBL1X
• Gene ID (NCBI): 6907
• RRID: AB_2199783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60907
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL1X is an essential subunit of the nuclear receptor corepressor (NCoR)-silencing mediator for retinoid and thyroid hormone receptors (SMRT) complex, the major TH receptor (TR) corepressor (CoR) involved in T3-regulated gene expression. TBL1X defects were recently associated with central hypothyroidism and hearing loss. (PMID: 30591955, PMID: 27603907)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Development; Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation.; Authors - Anna B Osipovich
human	WB,IP	Int J Biol Sci; TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma.; Authors - Hongjuan Xu ; KD Validated
mouse	WB,CoIP,ChIP	Nat Commun; The Fgf/Erf/NCoR1/2 repressive axis controls trophoblast cell fate; Authors - Andreas Lackner
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao