TBL2 Polyclonal antibody

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• TBL2 Polyclonal antibody (12488-1-AP)

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• KD/KO Validated

TBL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12488-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

transducin (beta) like 2, Transducin beta like protein 2, Transducin beta-like protein 2, UNQ563/PRO1125, WBSCR13

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  WB analysis using 12488-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 12488-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of human placenta using 12488-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 12488-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse placenta using 12488-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12488-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 12488-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of COLO 320 using 12488-1-AP

  IP result of anti-TBL2 (IP:12488-1-AP, 4ug; Detection:12488-1-AP 1:800) with COLO 320 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 12488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 12488-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 12488-1-AP (TBL2 antibody) at dilution of 1:25.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue, Caco-2 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human pancreas cancer tissue, human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12488-1-AP targets TBL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3200

  Product name: Recombinant human TBL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-446 aa of BC012938

  Sequence: SNGKYLATCADDRTIRIWSTKDFLQREHRSMRANVELDHATLVRFSPDCRAFIVWLANGDTLRVFKMTKREDGGYTFTATPEDFPKKHKAPVIDIGIANTGKFIMTASSDTTVLIWSLKGQVLSTINTNQMNNTHAAVSPCGRFVASCGFTPDVKVWEVCFGKKGEFQEVVRAFELKGHSAAVHSFAFSNDSRRMASVSKDGTWKLWDTDVEYKKKQDPYLLKTGRFEEAAGAAPCRLALSPNAQVLALASGSSIHLYNTRRGEKEECFERVHGECIANLSFDITGRFLASCGDRAVRLFHNTPGHRAMVEEMQGHLKRASNESTRQRLQQQLTQAQVTLGGPGAED

• Full Name: transducin (beta)-like 2
• Calculated Molecular Weight: 446 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC012938
• Gene Symbol: TBL2
• Gene ID (NCBI): 26608
• RRID: AB_2199923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4P3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Publications

What published studies show

TBL2 Polyclonal antibody (Cat# 12488-1-AP) has 6 citations published in Advanced Science, Annals of Surgical Oncology, Journal of Cellular Biochemistry, PLoS One, and Biochemical and Biophysical Research Communications. Research fields include spermatogenesis, breast cancer tumorigenesis via AKT signaling, lung adenocarcinoma, endoplasmic reticulum stress response, PERK-mediated stress signaling, and ribosome-associated translation regulation.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer; Authors - Xiuqing Lu
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
human	WB,IHC	Ann Surg Oncol; Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma; Authors - Keisuke Kosai ; KD Validated
	WB	J Cell Biochem; TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress.; Authors - Yoshinori Tsukumo
human	WB,IF	PLoS One; TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress.; Authors - Yoshinori Tsukumo ; KD Validated
human	WB,IF	Biochem Biophys Res Commun; The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit.; Authors - Yoshinori Tsukumo