TBX21,T-bet Polyclonal antibody

TBX21,T-bet Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB,IP,IHC,FC,ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 13700-1-AP

Synonyms

T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM

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Validation Data Gallery

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  WB analysis of A549 using 13700-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13700-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13700-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 13700-1-AP

  IP result of anti-TBX21,T-bet (IP:13700-1-AP, 4ug; Detection:13700-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13700-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13700-1-AP (TBX21,T-bet antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of NK92 using 13700-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human TBX21,T-bet (13700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A549 cells, mouse thymus tissue, RAW 264.7 cells
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: NK92

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

13700-1-AP targets TBX21,T-bet in WB,IP,IHC,FC,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBX21,T-bet fusion protein Ag4618
• Full Name: T-box 21
• Calculated Molecular Weight: 535 aa, 58 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC039739
• Gene Symbol: TBX21
• Gene ID (NCBI): 30009
• RRID: AB_10666436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Publications

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang ; KD Validated
human	WB	Int J Cardiol; Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.; Authors - Lu Yongguang Y
mouse	WB	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
rat	WB	Biomed Pharmacother; Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.; Authors - Yu Xia
human	WB	Front Pharmacol; Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.; Authors - Weizhen Li
mouse	IHC	Front Pharmacol; Cisplatin Promotes the Efficacy of Immune Checkpoint Inhibitor Therapy by Inducing Ferroptosis and Activating Neutrophils.; Authors - Ziwei Zhou