TBX21,T-bet Polyclonal antibody

TBX21,T-bet Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 13700-1-AP

Synonyms

T bet, T box 21, T box protein 21, T PET, TBET, TBLYM, TBX21, TBX21,T bet, TBX21,T-bet, Transcription factor TBLYM



Tested Applications

Positive WB detected inA549 cells, RAW 264.7 cells, mouse thymus tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13700-1-AP targets TBX21,T-bet in WB, IP, IHC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBX21,T-bet fusion protein Ag4618
Full Name T-box 21
Calculated molecular weight 535 aa, 58 kDa
Observed molecular weight 60-62 kDa
GenBank accession numberBC039739
Gene symbol TBX21
Gene ID (NCBI) 30009
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Protocols

Product Specific Protocols
WB protocol for TBX21,T-bet antibody 13700-1-APDownload protocol
IHC protocol for TBX21,T-bet antibody 13700-1-APDownload protocol
IP protocol for TBX21,T-bet antibody 13700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

Cancer Immunol Res

CD4 T Cells Require ICOS-Mediated PI3K Signaling to Increase T-Bet Expression in the Setting of Anti-CTLA-4 Therapy.

Authors - Hong Chen
humanWB

Oncol Lett

Effect of IL-17 in the development of colon cancer in mice.

Authors - Lijuan Yang
mouseWB

Dig Dis Sci

Inhibition of Pim-1 kinase ameliorates dextran sodium sulfate-induced colitis in mice.

Authors - Shen Yue-Ming YM
mouseWB

Infect Immun

B cell presentation of Chlamydia antigen selects out protective CD4γ13 T cells; implications for genital tract tissue resident memory lymphocyte clusters.

Authors - Raymond M Johnson
ratIHC

J Ethnopharmacol

Network pharmacology-based study on immunomodulatory mechanism of danggui-yimucao herb pair for the treatment of RU486-induced abortion.

Authors - Shi-Jie Bi
humanWB

Front Pharmacol

Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.

Authors - Weizhen Li