TBX21/T-bet Polyclonal antibody

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• TBX21/T-bet Polyclonal antibody (13700-1-AP)

TBX21/T-bet Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 13700-1-AP Copied!

Publications (42)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21

• 

  WB analysis using 13700-1-AP

  NK-92 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21/T-bet antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 13700-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21/T-bet antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 13700-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21/T-bet Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW 264.7 using 13700-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13700-1-AP (TBX21/T-bet antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of NK-92 using 13700-1-AP

  IP result of anti-TBX21/T-bet (IP:13700-1-AP, 4ug; Detection:13700-1-AP 1:5000) with NK-92 cells lysate 1400 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NK-92 using 13700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using TBX21,T-bet antibody (13700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NK-92 using 13700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using TBX21/T-bet antibody (13700-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of NK92 using 13700-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human TBX21/T-bet (13700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NK-92 cells, A549 cells, mouse thymus tissue, RAW 264.7 cells
• Positive IP detected in: NK-92 cells
• Positive IF/ICC detected in: NK-92 cells
• Positive FC (Intra) detected in: NK92

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13700-1-AP targets TBX21/T-bet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4618

  Product name: Recombinant human TBX21,T-bet protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC039739

  Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENF

• Full Name: T-box 21
• Calculated Molecular Weight: 535 aa, 58 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC039739
• Gene Symbol: TBX21/T-bet
• Gene ID (NCBI): 30009
• ENSEMBL Gene ID: ENSG00000073861
• RRID: AB_10666436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL17
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

Publications

What published studies show

The anti-T-bet antibody (Cat# 13700-1-AP) has 42 citations across journals including Cell, Nature Immunology, Advanced Science, Journal of Ethnopharmacology, International Immunopharmacology, and Frontiers in Pharmacology. Research fields span immunology (T cell differentiation, Th1/Th2 balance), oncology (glioblastoma, colorectal cancer, lymphoma), autoimmune/inflammatory diseases (asthma, SLE, atopic dermatitis), neurology (neuroinflammation, Alzheimer's disease), infectious disease, cardiology, and traditional pharmacology.

Automatically generated from the 42 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang ; KD Validated
mouse	WB	Nat Immunol; Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8(+) T cell fate.; Authors - Wei Hu
		Nat Immunol; The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates; Authors - Xin Zhao
mouse	IF	Adv Sci (Weinh); Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation; Authors - Mujie Liu
	IF	Adv Sci (Weinh); DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling-Mediated Anti-tumor Immunity; Authors - Kan Huang
mouse	IF	Adv Sci (Weinh); Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment; Authors - Yipeng Zhao