Tested Applications
| Positive WB detected in | NK-92 cells, A549 cells |
| Positive IP detected in | NK-92 cells |
| Positive IF/ICC detected in | NK-92 cells |
| Positive FC (Intra) detected in | NK92 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13700-1-AP targets TBX21/T-bet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4618 Product name: Recombinant human TBX21,T-bet protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC039739 Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENF Predict reactive species |
| Full Name | T-box 21 |
| Calculated Molecular Weight | 535 aa, 58 kDa |
| Observed Molecular Weight | 60-62 kDa |
| GenBank Accession Number | BC039739 |
| Gene Symbol | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| ENSEMBL Gene ID | ENSG00000073861 |
| RRID | AB_10666436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| IF protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| IP protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| WB protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBX21/T-bet Polyclonal antibody (Cat# 13700-1-AP) has 42 citations. These appear in journals including Cell, Nature Immunology, Advanced Science, Environmental Science & Technology, Journal of Translational Medicine, International Journal of Biological Macromolecules, Cancer Immunology Research, Chinese Medicine, Journal of Ethnopharmacology, Cancer Immunology & Immunotherapy, International Immunopharmacology, International Journal of Molecular Sciences, Frontiers in Pharmacology, Molecules, Biomedicine & Pharmacotherapy, and BMC Cancer. Research fields span immunology (Th1/Th2, Treg, T cell differentiation), cancer, inflammation, autoimmune disease, infectious disease, and neurology.
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.
| ||
Nat Immunol The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates | ||
Nat Immunol Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted CD8(+) T cell fate. | ||
Adv Sci (Weinh) Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment | ||
Adv Sci (Weinh) DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling-Mediated Anti-tumor Immunity | ||
Adv Sci (Weinh) Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation |
