Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16741-1-AP targets TBX3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, marmoset |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9955 Product name: Recombinant human TBX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 397-743 aa of BC025258 Sequence: TTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP Predict reactive species |
| Full Name | T-box 3 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 79 kDa |
| GenBank Accession Number | BC025258 |
| Gene Symbol | TBX3 |
| Gene ID (NCBI) | 6926 |
| RRID | AB_2200696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15119 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TBX3, also named as T-box protein 3, is a 743 amino acid protein, which contains 1 T-box DNA-binding domain. TBX3 may localize in the nucleus and is widely expressed in various tissues. TBX3 as a transcriptional repressor is involved in developmental processes and probably plays a role in limb pattern formation. TBX3 plays an important role on osteogenic differentiation and proliferation of human mesenchymal stem cell derived from adipose tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBX3 antibody 16741-1-AP | Download protocol |
| IP protocol for TBX3 antibody 16741-1-AP | Download protocol |
| WB protocol for TBX3 antibody 16741-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBX3 Polyclonal antibody (Cat# 16741-1-AP) has 10 citations. Journals include Nature Communications, Cell Death & Disease, International Journal of Molecular Medicine, Biological Research, Cell Signaling, Human Genomics, Experimental Cell Research, Genesis, Evidence-Based Complementary and Alternative Medicine, and Cell Molecular Gastroenterology and Hepatology. Research fields span cancer (thyroid, cholangiocarcinoma, bladder, ovarian, osteosarcoma), stem cell biology, congenital heart defects, psoriasis, and liver/cholestasis signaling.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors. | ||
Cell Death Dis Overexpression of TBX3 suppresses tumorigenesis in experimental and human cholangiocarcinoma | ||
Int J Mol Med Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/Akt‑dependent Tbx‑3 activation pathway. | ||
Biol Res Hsa_circ_0003611 hinders the transformation of mesenchymal stem cells into osteosarcoma cells through suppressing MYC by IGF2BP3 via m(6)A modification. | ||
Cell Signal A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells. | ||
Hum Genomics Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing. |
Reviews
What customers say
Customer feedback on this TBX3 antibody is mixed. One reviewer rated it 5 stars, praising its strong performance in Western Blot and Immunoprecipitation, noting it outperformed another antibody they had struggled with for months, and appreciating the included protocols. A second reviewer gave 1 star without written comments, having used it at 1:1000 dilution for Western Blot and Immunohistochemistry on human cell lines and mouse liver tissue. Overall, the antibody shows promise for IP and WB applications, though results may vary depending on the experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maggie (Verified Customer) (11-12-2025) | The TBX3 antibody works great! I have been trying to optimize protocols with a different antibody for months but even after all my experiments, the Proteintech antibody was much more promising for my IP and western blot protocols. I also really appreciate having the Proteintech protocols to refer to!
|
Li (Verified Customer) (12-03-2018) |
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