Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | rat lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13178-1-AP targets TBX4/5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3845 Product name: Recombinant human TBX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC027942 Sequence: MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKE Predict reactive species |
| Full Name | T-box 5 |
| Calculated Molecular Weight | 518 aa, 57 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC027942 |
| Gene Symbol | TBX5 |
| Gene ID (NCBI) | 6910 |
| RRID | AB_10638435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99593 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBX4/5 antibody 13178-1-AP | Download protocol |
| WB protocol for TBX4/5 antibody 13178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBX4/5 Polyclonal antibody (Cat# 13178-1-AP) has 12 citations published in journals including Nature Communications, European Respiratory Journal, Environmental Science & Technology, Molecular Biology and Evolution, Stem Cell Reports, Scientific Reports, iScience, Journal of Applied Toxicology, PeerJ, PLoS One, and American Journal of Medical Genetics A. Research fields span cardiac development, cardiomyocyte reprogramming, Holt-Oram syndrome, atrial fibrillation, neural differentiation, and congenital heart defects.
| Species | Application | Title |
|---|---|---|
Eur Respir J Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes. | ||
Nat Commun Gene-gene interactions between a LMNA variant and common polymorphisms drive early-onset atrial fibrillation. | ||
Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling. | ||
Mol Biol Evol Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates. | ||
Stem Cell Reports Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures. | ||
Sci Rep Ensuring expression of four core cardiogenic transcription factors enhances cardiac reprogramming. |
Reviews
What customers say
The single available review rates the product 5 out of 5 stars. The reviewer reported successful use in Western blot at a 1:100 dilution on fibroblasts overexpressing Tbx5, describing the antibody as "working good" for the application. Overall, the feedback is positive, indicating reliable performance for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Young-Jae (Verified Customer) (12-06-2018) | Working good for western blot
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