Telethonin Polyclonal antibody

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  WB analysis using 16104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16104-1-AP (Telethonin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16104-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16104-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16104-1-AP (Telethonin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Telethonin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16104-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TCAP, CMD1N

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Telethonin fusion protein Ag9014
• Full Name: titin-cap (telethonin)
• Calculated Molecular Weight: 167 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC012628
• Gene Symbol: TCAP
• Gene ID (NCBI): 8557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15273
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566).