TCEA3 Polyclonal antibody

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• TCEA3 Polyclonal antibody (17158-1-AP)

TCEA3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17158-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

TFIIS, TFIIS.H, TFIISH, Transcription elongation factor S-II protein 3

• 

  WB analysis using 17158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17158-1-AP (TCEA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 17158-1-AP

  IP result of anti-TCEA3 (IP:17158-1-AP, 4ug; Detection:17158-1-AP 1:500) with HeLa cells lysate 520ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 17158-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17158-1-AP (TCEA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17158-1-AP targets TCEA3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10404

  Product name: Recombinant human TCEA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-348 aa of BC041613

  Sequence: MGQEEELLRIAKKLEKMVARKNTEGALDLLKKLHSCQMSIQLLQTTRIGVAVNGVRKHCSDKEVVSLAKVLIKNWKRLLDSPGPPKGEKGEEREKAKKKEKGLECSDWKPEAGLSPPRKKREDPKTRRDSVDSKSSASSSPKRPSVERSNSSKSKAESPKTPSSPLTPTFASSMCLLAPCYLTGDSVRDKCVEMLSAALKADDDYKDYGVNCDKMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSADEPMTTFVLCNECGNRWKFC

• Full Name: transcription elongation factor A (SII), 3
• Calculated Molecular Weight: 348 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC041613
• Gene Symbol: TCEA3
• Gene ID (NCBI): 6920
• RRID: AB_2240352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75764
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TCEA3 Polyclonal antibody (Cat# 17158-1-AP) has 3 total citations. These citations appear in Journal of Cellular and Molecular Medicine, Journal of Gene Medicine, and Medical Science Monitor. The associated research fields include renal tubular epithelial cell autophagy, lung cancer tumorigenesis via tRNA-derived fragments, and gastric cancer growth inhibition through apoptosis induction. All three studies utilized the antibody for Western blot in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma
human	WB	J Gene Med; Oncogenic tRNA-derived fragment tRF-Leu-CAG promotes tumorigenesis of lung cancer via targeting TCEA3 and increasing autophagy; Authors - Fan Wu
human	WB	Med Sci Monit; TCEA3 Attenuates Gastric Cancer Growth by Apoptosis Induction.; Authors - Jia Li