TCEB3 Polyclonal antibody

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  WB analysis of mouse liver using 10502-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10502-1-AP

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10502-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10502-1-AP (TCEB3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10502-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCEB3 antibody (10502-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TCEB3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 10502-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SIII, MSTP059, Elongin-A, Elongin A, Elongin 110 kDa subunit

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10502-1-AP targets TCEB3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0778

  Product name: Recombinant human TCEB3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC002883

  Sequence: MAAESALQVVEKLQARLAANPDPKKLLKYLKKLSTLPITVDILAETGVGKTVNSLRKHEHVGSFARDLVAQWKKLVPVERNAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELERPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMYVDHYRSLEEDQEPIVSHQKPGKGHSNAFQDRLGASQERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGDLLPKVKEKGSNNLKTPEGKVKTNLDRKSLGSLPKVEETDMEDEF

• Full Name: transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC002883
• Gene Symbol: TCEB3
• Gene ID (NCBI): 6924
• RRID: AB_2201278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14241
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	FASEB J; TCEB3 initiates ovarian cancer apoptosis by mediating ubiquitination and degradation of MCL-1; Authors - Ying Cai ; KD Validated