TCERG1 Polyclonal antibody

TCERG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21858-1-AP

Synonyms

CA150, TAF2S, TCERG1, Transcription factor CA150

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Validation Data Gallery

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  WB analysis using 21858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21858-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21858-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21858-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21858-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21858-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21858-1-AP is TCERG1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCERG1 fusion protein Ag16482
• Full Name: transcription elongation regulator 1
• Calculated Molecular Weight: 1098 aa, 124 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC111727
• Gene Symbol: TCERG1
• Gene ID (NCBI): 10915
• RRID: AB_11183756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.