TCF12/HEB Polyclonal antibody

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  WB analysis using 14419-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14419-1-AP (TCF12/HEB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14419-1-AP

  WB result of TCF12/HEB antibody (14419-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCF12/HEB transfected HeLa cells.

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  WB analysis of Raji using 14419-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14419-1-AP (TCF12/HEB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14419-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14419-1-AP (TCF12/HEB antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14419-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14419-1-AP (TCF12/HEB antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TCF12/HEB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14419-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

TCF12, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Raji cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

14419-1-AP targets TCF12/HEB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5765

  Product name: Recombinant human TCF12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 358-706 aa of BC050556

  Sequence: SNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM

• Full Name: transcription factor 12
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC050556
• Gene Symbol: TCF12
• Gene ID (NCBI): 6938
• RRID: AB_2198838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99081
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Cell Biol; Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators; Authors - Bofeng Liu
human	IHC	Nat Commun; TCF12 is mutated in anaplastic oligodendroglioma.; Authors - Karim Labreche
mouse	IF	Development; A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.; Authors - Sisu Han
mouse	WB,IF	J Orthop Translat; TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4; Authors - Hui Zheng
mouse	WB,IHC,IF	Stem Cells; Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo.; Authors - Siqi Yi ; KD Validated
human	WB	iScience; Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.; Authors - Holly Holliday