TCF25 Polyclonal antibody

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  WB analysis using 30852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30852-1-AP (TCF25 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 30852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCF25 antibody (30852-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TCF25 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30852-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NULP1, FKSG26, hKIAA1049, Hulp1, KIAA1049

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30852-1-AP targets TCF25 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29375

  Product name: Recombinant human TCF25 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-251 aa of BC000959

  Sequence: MSRRALRRLRGEQRGQEPLGPGALHFDLRDDDDAEEEGPKRELGVRRPGGAGKEGVRVNNRFELINIDDLEDDPVVNGERSGCALTDAVAPGNKGRGQRGNTESKTDGDDTETVPSEQSHASGKLRKKKKKQKNKKSSTGEASENGLEDIDRILERIEDSTGLNRPGPAPLSSRKHVLYVEHRHLNPDTELKRYFGARAILGEQRPRQRQRVYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFE

• Full Name: transcription factor 25 (basic helix-loop-helix)
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC000959
• Gene Symbol: TCF25
• Gene ID (NCBI): 22980
• RRID: AB_3669765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BQ70
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development.