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KD/KO Validated

TCF3 Polyclonal antibody

TCF3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 21242-1-AP

Publications(15)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, CoIP, ChIP, ELISA, CUT&RUN

Synonyms

E2A, bHLHb21, Class B basic helix-loop-helix protein 21, E47, Immunoglobulin enhancer-binding factor E12/E47

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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WB analysis of Jurkat using 21242-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21242-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 21242-1-AP
WB result of TCF3 antibody (21242-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCF3 transfected Jurkat cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 21242-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 21242-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Jurkat using 21242-1-AP
IP result of anti-TCF3 (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 21242-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCF3 antibody (21242-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (15)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells, PC-3 cells, NIH/3T3 cells
Positive IP detected in	Jurkat cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21242-1-AP targets TCF3 in WB, IF/ICC, IP, CoIP, ChIP, ELISA, CUT&RUN applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15734 Product name: Recombinant human TCF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-480 aa of BC110580 Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ Predict reactive species
Full Name	transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated Molecular Weight	654 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC110580
Gene Symbol	TCF3
Gene ID (NCBI)	6929
RRID	AB_10755295
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15923
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for TCF3 antibody 21242-1-AP	Download protocol
IP protocol for TCF3 antibody 21242-1-AP	Download protocol
WB protocol for TCF3 antibody 21242-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IF	Nat Cell Biol Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators Authors - Bofeng Liu View Article
human	WB,CoIP	Cell Signal Targeting SDCBP2 in acute myeloid leukemia Authors - Yan Du View Article
human	WB	J Cell Mol Med The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. Authors - Meiju Zhang KD Validated View Article
mouse	WB	Mol Immunol Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development. Authors - Wei Liang View Article
rat	IP	Life Sci miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Authors - Ting Liu View Article
mouse	WB	Nat Commun A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation Authors - Jianfeng Wu View Article