TCF4/TCF7L2 Polyclonal antibody

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• TCF4/TCF7L2 Polyclonal antibody (13838-1-AP)

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13838-1-AP Copied!

Publications (47)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

TCF7L2, HMG box transcription factor 4, hTCF 4, hTCF-4, T cell factor 4

• 

  WB analysis of mouse liver using 13838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF4/TCF7L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 13838-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse placenta using 13838-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 13838-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 13838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 13838-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 13838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 13838-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 13838-1-AP

  IP result of anti-TCF4/TCF7L2 (IP:13838-1-AP, 4ug; Detection:13838-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4786

  Product name: Recombinant human TCF7L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC032656

  Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ

• Full Name: transcription factor 7-like 2 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 619 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC032656
• Gene Symbol: TCF4
• Gene ID (NCBI): 6934
• RRID: AB_2199812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQB0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.

Publications

What published studies show

Anti-TCF7L2 antibody (Cat# 13838-1-AP) has 47 citations published in journals including Nature Microbiology, Oncogene, FASEB Journal, Journal of Biological Chemistry, Cell Death Discovery, International Journal of Biological Sciences, and Biochemical Pharmacology. Research fields cover Wnt/β-catenin signaling, cancer (pancreatic, gastric, breast, ovarian, melanoma), neuroscience (Alzheimer's, depression, epilepsy, stroke), immunology, stem cell biology, metabolic disease, and fibrosis. Applications include WB, IF, IHC, ChIP, CoIP, and IP.

Automatically generated from the 47 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
mouse,human	WB,IHC	Drug Resist Updat; ATP-binding cassette subfamily A member 5 suppresses pancreatic ductal adenocarcinoma progression and chemoresistance by promoting β-catenin ubiquitin-dependent degradation.; Authors - Zhenchong Li
human	IHC,IF	Bone Res; Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis.; Authors - Pengqiang Lou
human	IF	Nat Microbiol; A single-cell transcriptomic atlas reveals senescence and inflammation in the post-tuberculosis human lung; Authors - Guoqiang Sun
human	WB,IF	J Control Release; Nanoceria drive stem cell hepatic differentiation and microenvironment remodeling for acute liver failure therapy.; Authors - Fenfang Li
human	WB	Pharmacol Res; Targeting aberrant glycosylation to modulate microglial response and improve cognition in models of Alzheimer's disease; Authors - Yue Wang