TCF4/TCF7L2 Polyclonal antibody

TCF4/TCF7L2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13838-1-AP

Synonyms

HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2



Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, human brain tissue, human heart tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, mouse placenta tissue, rat liver tissue, HEK-293 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13838-1-AP targets TCF4/TCF7L2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCF4/TCF7L2 fusion protein Ag4786
Full Name transcription factor 7-like 2 (T-cell specific, HMG-box)
Calculated Molecular Weight 619 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC032656
Gene Symbol TCF4
Gene ID (NCBI) 6934
RRIDAB_2199812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCF4/TCF7L2 antibody 13838-1-APDownload protocol
IP protocol for TCF4/TCF7L2 antibody 13838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal

Authors - Jing Wang
humanWB

Oncogene

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Authors - Kai Li
humanWB

Sci Rep

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Authors - Fengqin Dong
humanWB,IF,CoIP

Front Cell Dev Biol

Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin.

Authors - Nanlan Yu
humanWB

Mol Cancer Res

Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition.

Authors - Shuxuan Li
humanWB

J Biol Chem

HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis.

Authors - Yifan Chen