TCFL5 Polyclonal antibody

TCFL5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29404-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHA, Cha transcription factor, E2BP 1, E2BP1, Figlb, HPV 16 E2 binding protein 1, TCFL5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis of mouse testis using 29404-1-AP
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29404-1-AP (TCFL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 29404-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 29404-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	mouse testis tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

29404-1-AP targets TCFL5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13479 Product name: Recombinant human TCFL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-483 aa of BC046933 Sequence: VRVRLEDRFNSIPAEPPPAPRGPEPPEPGGALNNLVTLIRHPSELMNVPLQQQNKCTALVKNKTAATTTALQFTYPLFTTNACSTSGNSDLSQTQSSSNSCSVLEAAKHQDIGLPRAFSFCYQQEIESTKQTLGSRNKVLPEQVWIKVGEAALCKQALKRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQSGPQGGRSQRRERHNRMERDRRRRIRICCDELNLLVPFCNAETDKATTLQWTTAFLKYIQERHGDSLKKVLSTNSRYVMLWTCFLKMKSAVDGKNPQHAYRTANHKWLCLHEARSFAFEVSIWAHFQLFRVRIPEGFLSI Predict reactive species
Full Name	transcription factor-like 5 (basic helix-loop-helix)
Calculated Molecular Weight	483 aa, 52 kDa
Observed Molecular Weight	52-60 kDa
GenBank Accession Number	BC046933
Gene Symbol	TCFL5
Gene ID (NCBI)	10732
RRID	AB_2918297
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UL49
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.