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TCHP Polyclonal antibody

TCHP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25931-1-AP

Synonyms

Protein TCHP, TCHP, Tumor suppressor protein



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25931-1-AP is TCHP Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCHP fusion protein Ag23199
Full Name trichoplein, keratin filament binding
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC004285
Gene symbol TCHP
Gene ID (NCBI) 84260
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCHP antibody 25931-1-APDownload protocol
IHC protocol for TCHP antibody 25931-1-APDownload protocol
IF protocol for TCHP antibody 25931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Mol Life Sci

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Authors - Delowar Hossain
  • KD Validated