TCHP Polyclonal antibody

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  WB analysis of MCF-7 using 25931-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25931-1-AP (TCHP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25931-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 25931-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 25931-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25931-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25931-1-AP (TCHP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of MCF-7 using 25931-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TCHP antibody (25931-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

TCHP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25931-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Trichoplein keratin filament-binding protein, Protein TCHP, Mitostatin, Mitochondrial protein with oncostatic activity

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

25931-1-AP targets TCHP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23199

  Product name: Recombinant human TCHP protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-305 aa of BC004285

  Sequence: MALPTLPSYWCSQQRLNQQLARQREQEARLRQQWEQNSRYFRMSDICSSKQAEWSSKTSYQRSMHAYQREKMKEEKRRSLEARREKLRQLMQEEQDLLARELEELRLSMNLQERRIREQHGKLKSAKEEQRKLIAEQLLYEHWKKNNPKLREMELDLHQKHVVNSWEMQKEEKKQQEATAEQENKRYENEYERARREALERMKAEEERRQLEDKLQAEALLQQMEELKLKEVEATKLKKEQENLLKQRWELERLEEERKQMEAFRQKAELGRFLRHQYNAQLSRRTQQIQEELEADRRILQALLE

• Full Name: trichoplein, keratin filament binding
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC004285
• Gene Symbol: TCHP
• Gene ID (NCBI): 84260
• RRID: AB_2880300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BT92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Mol Life Sci; Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.; Authors - Delowar Hossain ; KD Validated
human	WB	Int J Mol Sci; Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization; Authors - Bo Li ; KD Validated
human	WB	J Cell Physiol; Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.; Authors - Min Huang ; KD Validated
human	WB,IF	Nat Commun; Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function; Authors - Jens S Andersen