TCN2 Monoclonal antibody

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  WB analysis of HUVEC using 66377-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66377-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TCN2 Monoclonal Antibody for WB, ELISA

Cat No. 66377-1-Ig

Clone No.2G6D2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

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Tested Applications

• Positive WB detected in: HUVEC cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66377-1-Ig targets TCN2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2825

  Product name: Recombinant human TCN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC011239

  Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW

• Full Name: transcobalamin II; macrocytic anemia
• Calculated Molecular Weight: 400 aa, 44 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC011239
• Gene Symbol: TCN2
• Gene ID (NCBI): 6948
• RRID: AB_2881754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20062
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)