TCOF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 11003-1-AP

Synonyms

treacle, MFD1

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Validation Data Gallery

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  WB analysis of Jurkat using 11003-1-AP

  WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.

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  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 11003-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (11003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

11003-1-AP targets TCOF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCOF1 fusion protein Ag1447
• Full Name: Treacher Collins-Franceschetti syndrome 1
• Calculated Molecular Weight: 97 kDa, 144 kDa, 152 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC014559
• Gene Symbol: TCOF1
• Gene ID (NCBI): 6949
• RRID: AB_2202516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Cell-fate determination by ubiquitin-dependent regulation of translation.; Authors - Achim Werner
human		Nat Commun; Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.; Authors - Stephen M Rawlinson
human	WB, IF	Proc Natl Acad Sci U S A; Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.; Authors - Alberto Ciccia ; KD Validated
human	WB	Elife; Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.; Authors - Achim Werner
human	WB, IF	Int J Mol Sci; TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.; Authors - Ahmed Hassan
human	WB,IF	Cancer Sci; TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.; Authors - Chaoshen Wu