TCP1 Monoclonal antibody, PBS Only

• 

  WB analysis using 68183-1-Ig (same clone as 68183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68183-1-Ig (TCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCP1 antibody (68183-1-Ig, Clone: 2C2F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TCP1 (68183-1-Ig, Clone:2C2F6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

TCP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68183-1-PBS

Clone No.2C2F6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CCT alpha, CCT1, CCTa, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

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Product Information

68183-1-PBS targets TCP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0389

  Product name: Recombinant human TCP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 277-553 aa of BC000665

  Sequence: KILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGA

• Full Name: t-complex 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000665
• Gene Symbol: TCP1
• Gene ID (NCBI): 6950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P17987
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.