TCP10L Polyclonal antibody

TCP10L Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13709-1-AP

Synonyms

C21orf77, PRED77, t complex 10 (mouse) like, T complex protein 10A 2, TCP10 like, TCP10A 2, TCP10L

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Validation Data Gallery

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  WB analysis of mouse testis using 13709-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13709-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13709-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13709-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13709-1-AP

  Immunofluorescent analysis of Hela cells, using 13709-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 13709-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13709-1-AP (TCP10L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13709-1-AP targets TCP10L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCP10L fusion protein Ag4646
• Full Name: t-complex 10 (mouse)-like
• Calculated Molecular Weight: 215 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC033855
• Gene Symbol: TCP10L
• Gene ID (NCBI): 140290
• RRID: AB_2255905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.