Anti-Mouse TCR beta (H57-597) Armenian Hamster Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• Anti-Mouse TCR beta (H57-597) Armenian Hamster Recombinant Antibody (65678-1-AR)

• 

  FC experiment of mouse splenocytes using 65678-1-AR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse TCR beta Hamster IgG2a RecAb (65678-1-AR, Clone: H57-597) or Armenian Hamster IgG Isotype Control (PIP) (65210-1-Ig, Clone: PIP), and FITC-conjugated Anti-Armenian Hamster IgG antibody. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65678-1-AR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse TCR beta Hamster IgG2a RecAb (65678-1-AR, Clone: H57-597) (red) or Armenian Hamster IgG Isotype Control (PIP) (65210-1-Ig, Clone: PIP) (blue), and FITC-conjugated Anti-Armenian Hamster IgG antibody. Cells were not fixed.

TCR beta Uni-rAb^® Recombinant Antibody for FC

Cat No. 65678-1-AR

Clone No.H57-597

Host / Isotype

Armenian Hamster / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Tcrb, H57-597, T cell receptor beta chain, TCRβ, Tib

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65678-1-AR targets TCR beta in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: T-cell receptor beta chain
• Gene Symbol: TCR beta
• Gene ID (NCBI): 21577
• RRID: AB_3743294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).