TDRD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TDRD3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13359-1-AP

Synonyms

TDRD3, tudor domain containing 3

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Validation Data Gallery

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  WB analysis of HEK-293 using 13359-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13359-1-AP (TDRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13359-1-AP

  WB result of TDRD3 antibody (13359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDRD3 transfected HEK-293 cells.

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  IP experiment of HeLa using 13359-1-AP

  IP result of anti-TDRD3 (IP:13359-1-AP, 3ug; Detection:13359-1-AP 1:1000) with HeLa cells lysate 3800ug.

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  IHC staining of mouse brain using 13359-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13359-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13359-1-AP

  Immunofluorescent analysis of HeLa cells using 13359-1-AP (TDRD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ChIP: See 1 publications below

Product Information

13359-1-AP targets TDRD3 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TDRD3 fusion protein Ag4174
• Full Name: tudor domain containing 3
• Calculated Molecular Weight: 651 aa, 73 kDa
• Observed Molecular Weight: 73 kDa, 83 kDa
• GenBank Accession Number: BC030514
• Gene Symbol: TDRD3
• Gene ID (NCBI): 81550
• RRID: AB_2202810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

Publications

Species	Application	Title
human	chIP	Theranostics; A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis.; Authors - Bing-Ling Peng