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  • KD/KO Validated

TDRKH specific Polyclonal antibody

TDRKH specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13528-1-AP

Synonyms

TDRD2, TDRKH, TDRKH specific, tudor and KH domain containing



Tested Applications

Positive WB detected inNCCIT cell, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13528-1-AP targets TDRKH specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDRKH specific fusion protein Ag4422
Full Name tudor and KH domain containing
Calculated Molecular Weight 606 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC032690
Gene Symbol TDRKH
Gene ID (NCBI) 11022
RRIDAB_2303299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Protocols

Product Specific Protocols
WB protocol for TDRKH specific antibody 13528-1-APDownload protocol
IHC protocol for TDRKH specific antibody 13528-1-APDownload protocol
IF protocol for TDRKH specific antibody 13528-1-APDownload protocol
IP protocol for TDRKH specific antibody 13528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.

Authors - Yu H Sun
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
mouseIF, WB

Nat Commun

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

Authors - Deqiang Ding
  • KO Validated
mouseIP

EMBO J

MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.

Authors - Chia-Ling Hsieh
mouseWB,IF

EMBO J

Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.

Authors - Jonathan P Saxe
  • KO Validated
mouseWB,IHC

Proc Natl Acad Sci U S A

Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.

Authors - Chen Chen C