Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31975-1-AP targets TEF in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37096 Product name: Recombinant human TEF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-185 aa of BC039258 Sequence: PVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARLDKEKGKEKLEEDEAAAASTMAVSASLMPPIWDKTIPYDGESFHLEYMDLDEFLLENGIPASPTHLAHNLLLPVAELEGKESASSSTASPPSSSTAIFQPSETVSSTESSLEKERETPSPIDPNCVEVDVNFNP Predict reactive species |
| Full Name | thyrotrophic embryonic factor |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC039258 |
| Gene Symbol | TEF |
| Gene ID (NCBI) | 7008 |
| RRID | AB_3742504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q10587 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEF (Thyrotroph Embryonic Factor) is a member of the PAR bZIP transcription factor family. Initially identified as being specifically expressed in pituitary thyrotroph cells during embryonic development, it regulates the transcription of the thyroid-stimulating hormone (TSH-β) gene. TEF activates target genes by binding to specific DNA sequences and is involved in cell cycle regulation, proliferation, and differentiation. Recent studies have revealed that TEF is significantly downregulated in various cancers, including bladder cancer, where it functions as a tumor suppressor by inhibiting tumor cell proliferation and metastasis through the AKT/FOXOs and Hippo signaling pathways, making it a potential tumor biomarker. In addition, as a regulator of circadian rhythms, polymorphisms in the TEF gene are associated with neuropsychiatric disorders such as Parkinson's disease, depression, and sleep disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEF antibody 31975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
