TERF2IP Polyclonal antibody, PBS Only

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• TERF2IP Polyclonal antibody, PBS Only (14595-1-PBS)

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  WB analysis of HEK-293 using 14595-1-AP (same clone as 14595-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14595-1-AP (same clone as 14595-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 14595-1-AP (same clone as 14595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 14595-1-AP (same clone as 14595-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 14595-1-AP (same clone as 14595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 14595-1-AP (same clone as 14595-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  IP result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug. This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 14595-1-AP (same clone as 14595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14595-1-AP (same clone as 14595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14595-1-PBS in a different storage buffer formulation.

TERF2IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14595-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Telomeric repeat-binding factor 2-interacting protein 1, RAP1 homolog, PP8000, hRap1, DRIP5

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Product Information

14595-1-PBS targets TERF2IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6130

  Product name: Recombinant human TERF2IP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-268 aa of BC005841

  Sequence: MAEAMDLGKDPNGPTHSSTLFVRDDGSSMSFYVRPSPAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEALAEASGDFISTQYILDCVERNERLELEAYRLGPASAADTGSEAKPGALAEGAAEPEPQRHAGRIAFTDADDVAILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGDAPVSPSSQKLKRKAEEDPEAADSGEPQNKRTPDLPEEEYVKEEIQENEEAVKKMLVEATREFEEVVVDE

• Full Name: telomeric repeat binding factor 2, interacting protein
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005841
• Gene Symbol: TERF2IP
• Gene ID (NCBI): 54386
• RRID: AB_10642697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYB0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)