TES Polyclonal antibody

TES Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 10258-1-AP

Synonyms

TES, Testin



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, MCF-7 cells, mouse testis tissue
Positive IP detected inK-562 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10258-1-AP targets TES in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TES fusion protein Ag0379
Full Name testis derived transcript (3 LIM domains)
Calculated molecular weight 48 kDa
Observed molecular weight 38-48 kDa
GenBank accession numberBC001451
Gene symbol TES
Gene ID (NCBI) 26136
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.

Protocols

Product Specific Protocols
WB protocol for TES antibody 10258-1-APDownload protocol
IF protocol for TES antibody 10258-1-APDownload protocol
IP protocol for TES antibody 10258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma.

Authors - Giedrius Steponaitis
mouseWB

Biol Reprod

The Aryl Hydrocarbon Receptor Is Important for Proper Seminiferous Tubule Architecture and Sperm Development in Mice.

Authors - Hansen Deborah A DA

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-07-2019)

The antibody works well. The first lane are cells expressing GFP-TES and the second lane is just wildtype cells. The antibody recognizes both endogenous and exogenous TES.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
TES Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10258-1-AP)