|Positive WB detected in||HEK-293 cells, K-562 cells, MCF-7 cells, mouse testis tissue|
|Positive IP detected in||K-562 cells|
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10258-1-AP targets TES in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TES fusion protein Ag0379|
|Full Name||testis derived transcript (3 LIM domains)|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||38-48 kDa|
|GenBank accession number||BC001451|
|Gene ID (NCBI)||26136|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-07-2019)
The antibody works well. The first lane are cells expressing GFP-TES and the second lane is just wildtype cells. The antibody recognizes both endogenous and exogenous TES.