Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11125-1-AP targets TESC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1596 Product name: Recombinant human TESC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC015221 Sequence: MGAAHSASEEVRELEGKTGFSSDQIEQLHRRFKQLSGDQPTIRKENFNNVPDLELNPIRSKIVRAFFDNRNLRKGPSGLADEINFEDFLTIMSYFRPIDTTMDEEQVELSRKEKLRFLFHMYDSDSDGRITLEEYRNVVEELLSGNPHIEKESARSIADGAMMEAASVCMGQMEPDQVYEGITFEDFLKIWQGIDIETKMHVRFLNMETMALCH Predict reactive species |
| Full Name | tescalcin |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC015221 |
| Gene Symbol | TESC |
| Gene ID (NCBI) | 54997 |
| RRID | AB_2201727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TESC antibody 11125-1-AP | Download protocol |
| IHC protocol for TESC antibody 11125-1-AP | Download protocol |
| WB protocol for TESC antibody 11125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TESC (Tescalcin) antibody (Cat# 11125-1-AP) has 15 citations across journals including Cell Stem Cell, Cancer Communications, Genome Biology, BMC Cancer, Journal of Neuroscience, Frontiers in Oncology, Oncology Reports, Endocrine, Gene, and Oncotarget. Research fields span cancer biology (hepatocellular, gastric, thyroid, esophageal, cholangio-, colorectal, and head & neck carcinomas; CML), neuroscience (Purkinje cell homeostasis, amyloid-β/Alzheimer's), cardiology (myocardial angiogenesis), and stem cell biology (lung epithelial differentiation).
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients.
| ||
Genome Biol Long non-coding RNA ROR decoys gene-specific histone methylation to promote tumorigenesis. | ||
Cancers (Basel) TESC Promotes TGF-α/EGFR-FOXM1-Mediated Tumor Progression in Cholangiocarcinoma. | ||
Oncol Rep Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells. | ||
Clin Exp Med TESC associated with poor prognosis enhances cancer stemness and migratory properties in liver cancer
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