TEX13A Polyclonal antibody

• 

  WB analysis of HL-60 using 18888-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spermatophore using 18888-1-AP

  mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18888-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18888-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 40x lens).

TEX13A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18888-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

testis expressed 13A, TEX13A

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-3 cells, K-562 cells, mouse spermatophore tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18888-1-AP targets TEX13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13468

  Product name: Recombinant human TEX13A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-409 aa of BC042547

  Sequence: MALRPEDPSSGFRHSNVVAFINEKMARHTKGPEFYLENISLSWEKVEDKLRAILEDSEVPSEVKEACTWGSLALGVRFAHRQAQLQRHRVRWLHGFAKLHKSAAQALASDLKKLREQQETERKEAASRLRMAQTSLVEVQKERDKELVSPHEWEQGAGWPGLATAGGVCTEGAAEEEEEAAVAAAGAAGGKGAEEEQRDVEVVAAPVEAMAPPVEAGAAPMETQFPHVEARAASMETTEKLERILLQLLGDADQEKYTYWGQKEGDLRSVETATSYFSGTTNPWSRASSEPLPVQLPASYSYSYSSPFSSFSDIPTISPPQATVTAPVPPQLPSDWEAFDTSLWSDGGPHRIDHQEHPRDRRYSEPHQQRPPVYRRPGDWDCPWCNAVNFSRRDTCFDCGKGIWLQKPH

• Full Name: testis expressed 13A
• Calculated Molecular Weight: 409 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC042547
• Gene Symbol: TEX13A
• Gene ID (NCBI): 56157
• RRID: AB_10641998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXU3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Lett; CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma; Authors - Ran Zhuo