TEX13A Polyclonal antibody

TEX13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18888-1-AP

Synonyms

testis expressed 13A, TEX13A

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Validation Data Gallery

• 

  WB analysis of HL-60 using 18888-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spermatophore using 18888-1-AP

  mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18888-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18888-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-3 cells, K-562 cells, mouse spermatophore tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18888-1-AP is TEX13A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX13A fusion protein Ag13468
• Full Name: testis expressed 13A
• Calculated Molecular Weight: 409 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC042547
• Gene Symbol: TEX13A
• Gene ID (NCBI): 56157
• RRID: AB_10641998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.