TEX14 Polyclonal antibody

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• TEX14 Polyclonal antibody (18351-1-AP)

TEX14 Polyclonal Antibody for IHC, ELISA

Cat No. 18351-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

• 

  WB analysis of mouse testis using 18351-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18351-1-AP (TEX14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18351-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18351-1-AP targets TEX14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13177

  Product name: Recombinant human TEX14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1149-1497 aa of BC040526

  Sequence: QPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPDSSFKEASCKTPKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFISVRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVEAFSQHHIDELPPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSKKEDSSMPLSKETEDLGEDTERAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQKVGEEKRKREDSITPERRKSEGVLGTSEEDELKSCFWKRLGWSESSRIIVLDQSDLSD

• Full Name: testis expressed 14
• Calculated Molecular Weight: 957aa,107 kDa; 1497aa,168 kDa
• Observed Molecular Weight: 180-200 kDa, 106 kDa
• GenBank Accession Number: BC040526
• Gene Symbol: TEX14
• Gene ID (NCBI): 56155
• RRID: AB_10641992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWB6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.

Publications

What published studies show

The TEX14 polyclonal antibody (Cat# 18351-1-AP) has 10 citations published in journals including Advanced Science, Cell Death & Disease, Developmental Cell, EMBO Journal, Cell Proliferation, Cells, International Journal of Molecular Sciences, FASEB Journal, eLife, and Methods in Molecular Biology. The research fields span reproductive biology, spermatogenesis, oocyte development, meiosis, cell division, and intercellular bridge formation.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Adv Sci (Weinh); Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice.; Authors - Rui Xu
mouse	IF	Cell Death Dis; Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.; Authors - Wen-Long Lei
human	IF	Dev Cell; Long-lived cytokinetic bridges coordinate sister-cell elimination in mouse embryos; Authors - Filip Vasilev
mouse	IF	EMBO J; Dynamic blebbing and absence of organelle transfer during mouse oocyte formation.; Authors - Eishi Aizawa
mouse	IF	Cell Prolif; YKT6 Is Essential for Male Fertility by Promoting Meiosis Progression During Spermatogenesis of Mice; Authors - Jie Cen
mouse	IF	Cells; The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication; Authors - Pablo López-Jiménez