Tested Applications
| Positive WB detected in | rat testis tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24585-1-AP targets TEX15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20170 Product name: Recombinant human TEX15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-97 aa of BC141841 Sequence: MPSDAKDSVNGDLLLNWTSLKNILSGLNASFPLHNNTGSSTVTTSKSIKDPRLMRREESMGEQSSTAGLNEVLQFEKSSDNVNSEIKSTPSNSASSS Predict reactive species |
| Full Name | testis expressed 15 |
| Calculated Molecular Weight | 2789 aa, 315 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC141841 |
| Gene Symbol | TEX15 |
| Gene ID (NCBI) | 56154 |
| RRID | AB_2879623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEX15 also named as Cancer/testis antigen 42 is a 2789 amino acid protein, which is expressed exclusively in testicular and cancerous tissues and may be involved in the development and metastasis of several different types of tumors. TEX15 exists as a 1279 amino acid protein in the mouse and rat testis. The molecular weight of mouse/rat TEX15 is 150 kDa (PMID: 26959739).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TEX15 antibody 24585-1-AP | Download protocol |
| IP protocol for TEX15 antibody 24585-1-AP | Download protocol |
| WB protocol for TEX15 antibody 24585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TEX15 Polyclonal antibody (Cat# 24585-1-AP) has 2 total citations. These appear in Fertility and Sterility and Toxicology Letters. The associated research fields are reproductive biology (subfertility and miRNA target gene expression in male patients) and toxicology (cadmium-induced spermatogenesis impairment via VDAC2/JNK/P53 signaling). Both citations used Western blot as the application, with one in human and one in mouse samples.
| Species | Application | Title |
|---|---|---|
Fertil Steril Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility. | ||
Toxicol Lett Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium. |
