TEX264 Recombinant antibody

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  WB analysis using 84946-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84946-5-RR (TEX264 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84946-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84946-5-RR against Human TEX264 were performed. The affinity constant is 0.254 nM.

TEX264 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84946-5-RR

Clone No.242500B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

242500B3, SIG11, TEG 264, Testis-expressed protein 264, TEX 264

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Tested Applications

• Positive WB detected in: Neuro-2a cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84946-5-RR targets TEX264 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TEX264 fusion protein Ag23027
• Full Name: testis expressed 264
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC008742
• Gene Symbol: TEX264
• Gene ID (NCBI): 51368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y6I9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.