Tested Applications
| Positive WB detected in | HEK-293 cells, C6 cells, C2C12 cells, Caco-2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82745-5-RR targets TFAM in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transcription factor A, mitochondrial |
| Calculated Molecular Weight | 246 aa, 29 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC126366 |
| Gene Symbol | TFAM |
| Gene ID (NCBI) | 7019 |
| RRID | AB_3743421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFAM antibody 82745-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TFAM Recombinant monoclonal antibody (250652B11), catalog number 82745-5-RR, has 7 citations published in Biomaterials, Antioxidants, J Ethnopharmacol, Int J Pharm, Foods, Appl Biochem Biotechnol, and Neuropeptides. Research fields include mitochondrial biogenesis and dynamics, diabetic osseointegration, skin photoaging, exercise-induced fatigue, spinal cord regeneration, cardiac ischemia-reperfusion injury, and chondrocyte function.
| Species | Application | Title |
|---|---|---|
Biomaterials A coenzyme-based "interface-welding" hydrogel coating for titanium implants promotes diabetic osseointegration by regulating mitochondrial dynamics. | ||
Antioxidants (Basel) Alpha-Ketoglutarate Attenuates UVB-Induced Skin Photoaging by Restoring Mitochondrial Redox Homeostasis. | ||
J Ethnopharmacol Atractylodes macrocephala Koidz. water extract alleviates exercise-induced fatigue by activating mitochondrial biogenesis via the PGC-1α/NRF1/TFAM axis. | ||
Int J Pharm Isoquercitrin-loaded adipose-derived stem cell exosomes synchronize immunomodulation and neurovascular remodeling to accelerate spinal cord regeneration. | ||
Foods Chemical Composition, Antioxidant Activity, Anti-Fatigue Function and Mechanism of Pomegranate Peel Polyphenols on Exercise-Induced Fatigue in Mice. | ||
Appl Biochem Biotechnol Electrical Vagus Nerve Stimulation Ameliorates Cardiac Ischemia and Reperfusion Injury by Improving Mitochondrial Biogenesis Through the SIRT1/PGC-1α Pathway. |
