TFAM Recombinant monoclonal antibody

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• TFAM Recombinant monoclonal antibody (82745-5-RR)

TFAM Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82745-5-RR

Clone No.250652B11

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

• 

  WB analysis using 82745-5-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 82745-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 82745-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82745-5-RR (TFAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, C6 cells, C2C12 cells, Caco-2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82745-5-RR targets TFAM in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• RRID: AB_3743421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00059
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF,WB	J Ethnopharmacol; Atractylodes macrocephala Koidz. water extract alleviates exercise-induced fatigue by activating mitochondrial biogenesis via the PGC-1α/NRF1/TFAM axis.; Authors - Guowei Ying
rat	WB	Biomaterials; A coenzyme-based "interface-welding" hydrogel coating for titanium implants promotes diabetic osseointegration by regulating mitochondrial dynamics.; Authors - Qiaojian Duan
		Antioxidants (Basel); Alpha-Ketoglutarate Attenuates UVB-Induced Skin Photoaging by Restoring Mitochondrial Redox Homeostasis.; Authors - Wenrui Zhang
		Int J Pharm; Isoquercitrin-loaded adipose-derived stem cell exosomes synchronize immunomodulation and neurovascular remodeling to accelerate spinal cord regeneration.; Authors - Wenjun Xia
		Neuropeptides; PACAP promotes mitochondrial biogenesis in rat primary chondrocytes via the SIRT1/PGC-1α pathway.; Authors - Liwei Xiong
mouse	WB	Foods; Chemical Composition, Antioxidant Activity, Anti-Fatigue Function and Mechanism of Pomegranate Peel Polyphenols on Exercise-Induced Fatigue in Mice.; Authors - Xing-Yu Ma