TFAP2C Polyclonal antibody

• Featured Product
• KD/KO Validated

TFAP2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14572-1-AP

Synonyms

ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1

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Validation Data Gallery

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  WB analysis of HeLa using 14572-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14572-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14572-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14572-1-AP

  IP result of anti-TFAP2C (IP:14572-1-AP, 4ug; Detection:14572-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• ChIP: See 3 publications below

Product Information

14572-1-AP targets TFAP2C in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAP2C fusion protein Ag5648
• Full Name: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC035664
• Gene Symbol: TFAP2C
• Gene ID (NCBI): 7022
• RRID: AB_2271424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,ChIP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
human	WB	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
human	chIP	Oncogene; TFAP2C regulates carbonic anhydrase XII in human breast cancer.; Authors - Christopher M Franke
human	IHC	Genes Immun; LncRNA sequencing reveals an essential role for the lncRNA-mediated ceRNA network in penile squamous cell carcinoma; Authors - Jian Cao
human	WB	Front Oncol; TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling.; Authors - Xingkang Jiang
human	WB,IHC	Cancers (Basel); TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB; Authors - Ji Xing ; KD Validated