TFAP2C/AP2 gamma Polyclonal antibody

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• TFAP2C/AP2 gamma Polyclonal antibody (14572-1-AP)

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• KD/KO Validated

TFAP2C/AP2 gamma Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14572-1-AP Copied!

Publications (17)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, chIP, ELISA
For FC, select conjugated version.

Synonyms

TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma

• 

  WB analysis of HeLa using 14572-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 14572-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14572-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14572-1-AP

  IP result of anti-TFAP2C (IP:14572-1-AP, 4ug; Detection:14572-1-AP 1:1000) with HeLa cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14572-1-AP targets TFAP2C/AP2 gamma in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5648

  Product name: Recombinant human TFAP2C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-450 aa of BC035664

  Sequence: MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK

• Full Name: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC035664
• Gene Symbol: TFAP2C
• Gene ID (NCBI): 7022
• RRID: AB_2271424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92754
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research.

Publications

What published studies show

The TFAP2C antibody (Cat# 14572-1-AP) has 17 citations published across 14 journals, including Nature Cell Biology, Nature Communications, Molecular Cancer, Oncogene, Cancer Letters, and Cell Reports. Research fields span cancer biology (breast, ovarian, prostate, colorectal, lung, bladder, and penile cancers), ferroptosis regulation, transcriptional control, signaling pathways (PI3K/AKT, Wnt/β-catenin, NF-κB), and early embryogenesis/PRC2 function. Applications include WB, ChIP, IHC, and IF in human and mouse samples.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,ChIP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
		Nat Cell Biol; Decoding stage-specific functions of PRC2 in early embryogenesis uncovers roles in preimplantation development and primordial germ cell fate.; Authors - Chengjie Zhou
human	IHC	Nat Commun; Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment; Authors - Xiaojian Lu
human	WB	Cancer Lett; The Megacomplex protects ER-alpha from degradation by Fulvestrant in epithelial ovarian cancer; Authors - Sushil Kumar Jaiswal
human	WB	Cancer Lett; TFAP2C-DDR1 axis regulates resistance to CDK4/6 inhibitor in breast cancer; Authors - Muhammad Jameel Mughal
human	ChIP	Oncogene; TFAP2C protects against ferroptosis in ovarian cancer through the KEAP1-NRF2 axis by recruiting HDAC1/2.; Authors - Guiyun Cheng