TFAP2C/AP2 gamma Recombinant monoclonal antibody

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  WB analysis using 86365-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86365-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86365-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TFAP2C antibody (86365-1-RR, Clone: 250904E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TFAP2C/AP2 gamma Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86365-1-RR

Clone No.250904E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma

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Tested Applications

• Positive WB detected in: MCF-7 cells, PC-3 cells, SK-BR-3 cells, A431 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86365-1-RR targets TFAP2C/AP2 gamma in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5648

  Product name: Recombinant human TFAP2C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-450 aa of BC035664

  Sequence: MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK

• Full Name: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC035664
• Gene Symbol: TFAP2C
• Gene ID (NCBI): 7022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92754
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research.