TFAP4 Polyclonal antibody

TFAP4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12017-1-AP

Synonyms

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

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Validation Data Gallery

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  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12017-1-AP

  IP result of anti-TFAP4 (IP:12017-1-AP, 4ug; Detection:12017-1-AP 1:1000) with HeLa cells lysate 1360 ug.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, NCI-H1299 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12017-1-AP targets TFAP4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAP4 fusion protein Ag2633
• Full Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010576
• Gene Symbol: TFAP4
• Gene ID (NCBI): 7023
• RRID: AB_2199571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Agric Food Chem; Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling.; Authors - Peng Yang
human	WB	J Agric Food Chem; Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway.; Authors - Peng Yang
human	WB	Invest Ophthalmol Vis Sci; A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway.; Authors - Ruonan Wang