TFAP4 Polyclonal antibody

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• TFAP4 Polyclonal antibody (12017-1-AP)

TFAP4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 12017-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ChIP, ELISA, CUT&Tag

Synonyms

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

• 

  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 12017-1-AP

  IP result of anti-TFAP4 (IP:12017-1-AP, 4ug; Detection:12017-1-AP 1:1000) with HeLa cells lysate 1360 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, NCI-H1299 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12017-1-AP targets TFAP4 in WB, IHC, IP, ChIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2633

  Product name: Recombinant human TFAP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC010576

  Sequence: MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

• Full Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010576
• Gene Symbol: TFAP4
• Gene ID (NCBI): 7023
• RRID: AB_2199571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01664
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Anti-TFAP4 antibody (Cat# 12017-1-AP) has 12 citations published in journals including Cell Stem Cell, Science Advances, Frontiers in Immunology, Journal of Agricultural and Food Chemistry, Cancer Gene Therapy, Investigative Ophthalmology & Visual Science, Journal of Biochemical and Molecular Toxicology, Archives of Biochemistry and Biophysics, Experimental Cell Research, and Journal of Molecular Histology. Research fields span breast cancer metastasis, axon regeneration, leukemia, renal fibrosis, colorectal/endometrial/prostate cancer, retinal neovascularization, and cancer immunology.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CUT&Tag	Cell Stem Cell; Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis.; Authors - Xinxin Rao
mouse	WB,IHC	Sci Adv; Transcription factor activator protein 4 (AP4)-mediated intrinsic control of axon regeneration.; Authors - Ngan Pan Bennett Au ; KD Validated
human	WB	Sci Adv; Genome-wide CRISPR screen identifies IRF1 and TFAP4 as transcriptional regulators of Galectin-9 in T cell acute lymphoblastic leukemia; Authors - Caroline R M Wiggers ; KO Validated
human,mouse	WB,IHC,ChIP	Front Immunol; Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease; Authors - Enyi Zhu
human	WB	J Agric Food Chem; Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling.; Authors - Peng Yang
human	WB	J Agric Food Chem; Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway.; Authors - Peng Yang