TFE3 Monoclonal antibody

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• TFE3 Monoclonal antibody (67319-1-Ig)

TFE3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67319-1-Ig Copied!

Clone No. 1E3D7

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

1E3D7, bHLHe33, Class E basic helix-loop-helix protein 33, RCCP2, TFE 3

• 

  WB analysis using 67319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of C2C12 using 67319-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 67319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 67319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 67319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue
• Positive IHC detected in: human kidney tissue, human cervical cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6320

  Product name: Recombinant human TFE3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC026027

  Sequence: MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN

• Full Name: transcription factor binding to IGHM enhancer 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026027
• Gene Symbol: TFE3
• Gene ID (NCBI): 7030
• RRID: AB_2882579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P19532
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TFE3 Monoclonal antibody (1E3D7), catalog number 67319-1-Ig, has 1 total citation. The citation appears in Cell Communication and Signaling (2025), a study investigating how TGF-β2 increases eHSP90α secretion via the secretory autophagy pathway. The research field is cell signaling and autophagy. The antibody was used in Western blot (WB) with human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Commun Signal; TGF-β2 increases eHSP90α secretion via upregulating secretory autophagy pathway.; Authors - Jing Li ; KD Validated