TFE3 Monoclonal antibody

TFE3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3D7

Cat no : 67319-1-Ig

Synonyms

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

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Validation Data Gallery

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  WB analysis using 67319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 67319-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 67319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IHC staining of human malignant melanoma using 67319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 10x lens)..

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Tested Applications

• Positive WB detected in: HEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue
• Positive IHC detected in: human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFE3 fusion protein Ag6320
• Full Name: transcription factor binding to IGHM enhancer 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026027
• Gene Symbol: TFE3
• Gene ID (NCBI): 7030
• RRID: AB_2882579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.